Jackie Estacado, in the two years since the events of the first game, has become the don of the Franchetti crime family. Though he still wields the Darkness, a malevolent force that has given him supernatural powers in its quest for death and destruction, Jackie has tempered the entity, thanks to guidance from the estranged occultist Johnny Powell. Jackie still struggles with the death of his girlfriend, Jenny Romano, which the Darkness had prevented him from stopping. Now faced with yet another life or death situation facing new, yet unknown enemies... He is forced to rely on the power of The Darkness to survive. The question is... Will he learn to tame the power he has been granted... or will he become an empty shell, and give in to the evil tendencies of the Darkness?